Description
High Density Lipoprotein (HDL) is also a spherical particle with diameter of about 10 nm (Figure 1). HDL contains the Apoliprotein AI and AII molecules. HDL and LDL cholesterol levels in the blood are important indicators of many disease states. High blood levels of LDLs are associated with health problems and cardiovascular disease. For this reason, LDL is often referred to as the "bad cholesterol." LDL particles that accumulate within arteries can form plaques over time, which can increase chances of a stroke, heart attack, or vascular disease. HDL particles are able to remove cholesterol from within arteries and transport it back to the liver for re-utilization or excretion, which is the main reason why the cholesterol carried within HDL particles is sometimes called "good cholesterol." Monitoring circulatory levels of different lipoproteins is critical to the diagnosis of lipid transport disorders such as atherosclerosis.The HDL Purification Kit uses Dextran Sulfate to selectively precipitate HDL. The kit allows for the purification of HDL without the need for ultracentrifugation. The lipoprotein particles are highly purified through a series of precipitation and low speed centrifugation steps. Each kit provides sufficient reagents to perform up to 10 preps, and each preparation can purify up to 10 mL of serum or plasma samples with a yield of ~4500 ug of HDL per mL for human samples (expected yield will vary by species)